Penguin Musume♥Heart Special

A strong fighter from China. She made a promise with Kujira at a younger age, (who was still under the delusion she was a boy) that if Chi-Cha could defeat her, Kujira would become her husband. While Kujira has since realized she was a girl and has made it very clear to all, Chi-Cha still remains clueless and vows to make her her husband someday.

Cha Chi

A girl who is skilled in Etorofu martial arts. She was raised as a boy by her father to become a strong fighter, and whilst she has since realised she is actually a girl, her father hasn't. She is generally hounded by Penguin due to a likeness to one of her favourite anime stars.  (Source: Wikipedia)

Kujira Etorofu

A calm girl who always remains composed with a smile on her face, no matter what happens. She lives near the shrine and works as a shrine maiden.

Nene Kurio

The lead character who is obsessed with manga and anime. Whenever she cosplays as someone, she obtains the abilities of that outfit. She is part of a very rich family and is nicknamed Penguin due to her penguin-like hairstyle and her surname which means "South Pole".

Sakura Nankyoku

Based on a real person. Introducing new penguin musume goods at the end of each episode.

Miho-chan-sensei

Works as the Nankyoku family's butler. He has a wide variety of skills used to serve Penguin and Kaede, and occasionally dresses in women's clothing.  (Source: Wikipedia)

Sebastian

Penguin's rival, represented by a polar bear. A tragic moment in her past has caused her to despise the Nankyoku company, and constantly seeks to overwhelm it, normally by building something bigger than what they have. Later on though, she becomes bankrupt and has to use tattered clothing whilst maintaining her lady-like dignity. She is also referred to as 'Shirokuma' (a literal translation of Whitebear) and is sometimes called 'Kuma-san' by Penguin.  (Wiki)

Mary Chupacabra W. Whitebear